Journal: Nature Communications

Article Title: Hypoxia-tropic delivery of nanozymes targeting transferrin receptor 1 for nasopharyngeal carcinoma radiotherapy sensitization

doi: 10.1038/s41467-025-56134-z

Figure Lengend Snippet: a Schematic representation of CSPs upregulated in hypoxic NPC identified through DGE analysis among a total of 1442 CSPs. b Left: Venn diagram illustrating the intersection of CSPs upregulated in tumor samples of the three cohorts: GSE12452 (log 2 (fold change) >1 and P < 0.01), GSE53819 (log 2 (fold change) >1 and P < 0.01), and GSE61218 (log 2 (fold change) >1 and P < 0.01). Right: Venn diagram illustrating the intersection of CSPs that are upregulated in tumor samples with high hypoxia scores of the GSE102349 cohort (log 2 (fold change) >10 and P < 0.01). c Distribution of TfR1 mRNA abundance in tumor and normal samples from different cohorts: GSE12452 (normal, n = 10; tumor, n = 31), GSE53819 (normal, n = 18; tumor, n = 18), and GSE61218 (normal, n = 6; tumor, n = 10). d Distribution of TFRC mRNA abundance in tumor samples from the GSE102349 cohort ( n = 113) with high or low hypoxia scores. e Representative images of IHC staining showing TfR1 protein expression in clinical primary NPC tissues and NNE tissues collected from multiple patients. Scale bar, 50 μm. f Distribution of TfR1 IHC staining scores in NPC ( n = 174) and NNE ( n = 98) tissues. g Distribution of TfR1 IHC staining scores in NPC and matched adjacent non-cancerous tissues ( n = 117). h IF staining analysis in NPC tissues depicting the co-expression of HIF-1α and TfR1. Scale bar, 50 μm. i Spearman correlation between HIF-1α expression and TfR1 expression based on h ( n = 40 random regions from different tissues). The box plots in c , d , and f show the median ±1 quartile, with whiskers extending to the minimum or maximum values within 1.5 times the interquartile range from the box boundaries. Mann–Whitney test in c , d , and f and Wilcoxon matched-pairs signed rank test in g were used to calculate P values. Source data are provided as a Source Data file.

Article Snippet: Subsequently, 300 µL of 5% skimmed milk was added to each well and incubated at 37 °C for 2 h. After further washing, each well was incubated with 100 µL of TfR1 solution (SinoBiological, catalog no. 11020-H07H, 2 µg/mL) at 37 °C for 2 h. After another round of washing, each well was incubated with 100 µL of anti-TfR1 antibody (SinoBiological, catalog no. 11020-MM02, 1 µg/mL) at 37 °C for 2 h. Following additional washes, 100 µL of HRP-conjugated anti-mouse IgG (GE Healthcare, catalog no. NA931, 1:5000) was added and incubated at 37 °C for 1 h. Finally, 100 µL of one-component TMB chromogenic substrate (InnoReagents, catalog no. TMB-S-004) was added to each well.

Techniques: Immunohistochemistry, Expressing, Staining, MANN-WHITNEY

Journal: American Journal of Cancer Research

Article Title: Transferrin receptor 1 in cancer: a new sight for cancer therapy

doi:

Figure Lengend Snippet: Location of TFR1 in three cancer cell lines. Immunofluorescence analysis of TFR1 in A431, U2OS and U251 MG was obtained from The Human Protein Atlas database. TFR1 (Green fluorescence) expressed in vesicles (A), endosomes and lysosomes (B).

Article Snippet: Immunohistochemistry analysis of TFR1 in various normal tissues and tumor tissues was obtained from The Human Protein Atlas database.

Techniques: Immunofluorescence, Fluorescence

Journal: American Journal of Cancer Research

Article Title: Transferrin receptor 1 in cancer: a new sight for cancer therapy

doi:

Figure Lengend Snippet: TFR1 expression in various normal tissues and tumor tissues. Immunohistochemistry analysis of TFR1 in various normal tissues and tumor tissues was obtained from The Human Protein Atlas database. TFR1 is overexpression in multiple tumor tissues compared to normal tissues.

Article Snippet: Immunohistochemistry analysis of TFR1 in various normal tissues and tumor tissues was obtained from The Human Protein Atlas database.

Techniques: Expressing, Immunohistochemistry, Over Expression

Journal: American Journal of Cancer Research

Article Title: Transferrin receptor 1 in cancer: a new sight for cancer therapy

doi:

Figure Lengend Snippet: TFR1 and its regulatory effects on tumors Iron concentration changes significantly in cancer cells. TFR1 binds mutated HFE to promote iron intake. Iron concentration influences TFRC post-transcription by regulating the binding of IRP1 and IRP2. Meanwhile, iron concentration affects the activities of c-Jun, cyclin D and C-myc. Some miRNAs also influence TFRC transcription. These bioprocesses contribute to cell progression and tumor growth.

Article Snippet: Immunohistochemistry analysis of TFR1 in various normal tissues and tumor tissues was obtained from The Human Protein Atlas database.

Techniques: Concentration Assay, Binding Assay

Journal: American Journal of Cancer Research

Article Title: Transferrin receptor 1 in cancer: a new sight for cancer therapy

doi:

Figure Lengend Snippet: TFR1 interaction network. Based on the search tool of the European Molecular Biology Laboratory for retrieving interacting genes/proteins, an interaction network with confidence levels of > 0.7 is exhibited for genetically interacting, possibly TFR1-related proteins. A thicker line represents a stronger interaction.

Article Snippet: Immunohistochemistry analysis of TFR1 in various normal tissues and tumor tissues was obtained from The Human Protein Atlas database.

Techniques:

Journal: American Journal of Cancer Research

Article Title: Transferrin receptor 1 in cancer: a new sight for cancer therapy

doi:

Figure Lengend Snippet: Future directions for TFR1 research in tumor. The direct effects of TFR1 expression on carcinogenesis remains further study. TFR1’s functions and mechanisms in cancer cell ferroptosis are still unclear. Nanomedicine combine with magnetic fields targeting TFR1 will be a potential cancer therapy.

Article Snippet: Immunohistochemistry analysis of TFR1 in various normal tissues and tumor tissues was obtained from The Human Protein Atlas database.

Techniques: Expressing